Journal Basic Info

  • Impact Factor: 1.989**
  • H-Index: 6
  • ISSN: 2637-4625
  • DOI: 10.25107/2637-4625
**Impact Factor calculated based on Google Scholar Citations. Please contact us for any more details.

Major Scope

  •  Cardiothoracic Surgery
  •  Colorectal Surgery
  •  Vascular Surgery
  •  Cardiac Surgery
  •  Minimal Invasive Surgery
  •  Aesthetic & Cosmetic Surgery
  •  Orthopaedic Surgery
  •  Ophthalmology

Abstract

Citation: World J Surg Surg Res. 2019;2(1):1105.DOI: 10.25107/2637-4625.1105

Stabilization of Molecular Structure of the Proteins and Lipids of the Erythrocyte Membranes by Means Magnetite Nanoparticles (ICNB)

Andrey Belousov, Elena Malygon, Vadim Yavorskiy and Ekateryna Belousova

Department of Anesthesiology, Laboratory of Applied Nanotechnology of Belousov, Ukraine
Kharkov Medical Academy of Postgraduate Education, Ukraine
Kharkov Regional Center of Blood Service, Ukraine

*Correspondance to: Andrey Belousov 

 PDF  Full Text Research Article | Open Access

Abstract:

This study was devoted to the learning changes in the structure of erythrocyte membranes at the level of molecular bonds during their storage at a positive temperature by means method of Infrared Spectroscopy (IR spectroscopy). Objects of research were Red Blood Cells (RBC) into bags containing preservative CPD and RBCs into bags containing preservative CPDA-1. As membrane protective used saline which had previously been treated with magnetite nanoparticles (ICNB) by the Belousov's method. The physiological solution that was treated with nanoparticles was added to the preserved RBCs according to the developed method. Sample of control was the addition of intact saline. Analysis of changes occurring in the IR spectra of samples of control and test in the CPD medium was showed that during the first 28 days storage of: 1. In the suspension of control of the RBCs, noticeable destructive changes in the molecular structure membrane of erythrocytes at the 14th day storage begin. After three weeks, the molecular structure membrane of erythrocytes is completely destroyed; 2. In the sample of test there was a weakening and rupture of molecular bonds only at the 28th day storage of RBCs. Complete destruction of the structure of membrane of erythrocytes occurs at the 35th day storage. Analysis of changes occurring in the IR spectra of samples of control and test in the CPDA-1 medium was showed that during 49 days storage of: 1. In the suspension of control of RBCs noticeable destructive changes in the molecular structure begins in four weeks, and after six weeks storage the molecular structure of erythrocytes membrane are completely destroyed; 2. In the sample of test, a significant weakening of intra-and intermolecular bonds in the structure of erythrocytes membrane occurs after six weeks. However, the complete destruction of the structure is not observed. After seven weeks storage of erythrocytes obvious violations of the molecular structure of lipids and proteins that make up the RBCs are visible but some of the strongest compounds still remain. In general, the results clearly showed that the presented method of application of nanotechnology significantly increases the storage time of RBCs in different versions of preservatives due to mechanisms to reduce violations of the molecular structure of proteins and lipids in the erythrocyte membranes. Presented method of application of nanotechnology is not only safe for use in practice in the Blood Service, Transfusiology and Hematology, but also is the most promising innovation project.

Keywords:

Nanotechnology; Preserved red blood cells; Magnetite nanoparticles; Method of infrared spectroscopy; Storage time of RBCs

Cite the Article:

Belousov A, Malygon E, Yavorskiy V, Belousova E. Stabilization of Molecular Structure of the Proteins and Lipids of the Erythrocyte Membranes by Means Magnetite Nanoparticles (ICNB). World J Surg Surgical Res. 2019; 2: 1105.

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