Journal Basic Info
- Impact Factor: 1.989**
- H-Index: 6
- ISSN: 2637-4625
- DOI: 10.25107/2637-4625
Major Scope
- Reconstructive Surgery
- Gastroenterological Surgery
- Urological Surgery
- Ophthalmology
- Hepatology
- Surgery & Surgical Research
- Surgical Procedures
- Cardiovascular Surgery
Abstract
Citation: World J Surg Surg Res. 2018;1(1):1025.DOI: 10.25107/2637-4625.1025
Synovial Fluid Analysis in Patients with Osteoarthritis Requiring Total Joint Arthroplasty
Elissa S. Davis, Christopher Wanderling, Daniel R. Schmitt, Jeffrey Liles, Kevin Galicia, Debra Hoppensteadt, Jawed Fareed and William Hopkinson
Department of Orthopaedic Surgery and Rehabilitation, Loyola University Medical Center, USA
Loyola University of Chicago, Stritch School of Medicine, Maywood, IL
Department of Pathology, Loyola University Medical Center, USA
*Correspondance to: Elissa S Davis
PDF Full Text Research Article | Open Access
Abstract:
Background: Osteoarthritis (OA) is a progressive disease characterized by degeneration of the articular cartilage in joints. Currently, there are no preventative treatments available for OA, which has resulted in an increasingly heavy burden on the country’s healthcare system as the population continues to age. Despite the increasing emphasis on preventative health care overall in medicine, there have been minimal recent improvements in the prevention or management of OA.
Objective: To profile total protein content, CRP, IL-1b, MP-TF and PAI-1 in patients who suffer from OA that require total joint arthroplasty (TJA) procedure.
Method: Synovial fluid (SF) samples were taken perioperatively from 62 patients undergoing either total knee arthroplasty (TKA) or total hip arthroplasty (THA) procedures. The SF was then purified by centrifugation. Samples were aliquoted and stored at 80°C. Samples were analyzed for total protein by photo spectrometry and ELISA assays were used to analyze CRP, IL-1b, MP-TF and PAI-1 levels.
Results: The mean total protein load was 19.27 mg/ml ± 13.89 mg/ml. The mean values for these biomarkers were: CRP 1.54 ng/ml ± 1.86 ng/ml, IL-1b 0.19 pg/ml ± 0.18 pg/ml, MP-TF 14.17 pg/ml ± 19.94 pg/ml and PAI-1 19.87 ng/ml ± 6.29 ng/ml.
Conclusion: This novel study investigating the content of SF demonstrates that these biomarkers are present at detectable levels. These results may help better define the pathogenesis of OA and aid in the development of targeted therapies against the inflammatory pathway.
Keywords:
Osteoarthritis; Arthroplasty; Synovial fluid; Inflammation
Cite the Article:
Davis ES, Wanderling C, Schmitt DR, Liles J, Galicia K, Hoppensteadt D, et al. Synovial Fluid Analysis in Patients with Osteoarthritis Requiring Total Joint Arthroplasty. World J Surg Surgical Res. 2018; 1: 1025.